| 9/3/2016 | Northern Iowa (5-6) | 25 | at | Iowa State | 20 | MVC (1AA) | ||
| 9/10/2016 | Iowa State | 3 | at | Iowa (8-5) | 42 | at B10 | ||
| 9/17/2016 | Iowa State | 20 | at | TCU (6-7,4-5) | 41 | |||
| 9/24/2016 | San Jose State (4-8) | 10 | at | Iowa State | 44 | MW | ||
| 10/1/2016 | Baylor (7-6,3-6) | 45 | at | Iowa State | 42 | |||
| 10/8/2016 | Iowa State | 31 | at | Oklahoma State (10-3,7-2) | 38 | |||
| 10/15/2016 | Iowa State | 6 | at | Texas (5-7,3-6) | 27 | |||
| 10/29/2016 | Kansas State (9-4,6-3) | 31 | at | Iowa State | 26 | |||
| 11/3/2016 | Oklahoma (11-2,9-0) | 34 | at | Iowa State | 24 | |||
| 11/12/2016 | Iowa State | 31 | at | Kansas (2-10,1-8) | 24 | |||
| 11/19/2016 | Texas Tech (5-7,3-6) | 10 | at | Iowa State | 66 | |||
| 11/26/2016 | West Virginia (10-3,7-2) | 49 | at | Iowa State | 19 |
| vs Iowa State | vs Other Opponents | Iowa State compared to OOs | |
| Record: | 9-3 | 73-65 | |
| Points Scored: | 31.33 | 32.33 | 1.00 |
| Points Allowed: | 27.67 | 28.86 | -1.19 |
| Margin: | 3.66 | 3.47 | -0.19 |
| Total Points: | 59.00 | 61.19 | -2.19 |