| 8/28/2010 | Charleston WV Golden Eagles 12 @ Tusculum 24 | |
| 9/4/2010 | Tusculum 16 @ Findlay Oilers 3 | |
| 9/11/2010 | Tusculum 54 @ Western Carolina Catamounts 30 | |
| 9/18/2010 | Urbana Blue Knights 36 @ Tusculum 39 | |
| 10/2/2010 | Tusculum 39 @ Mars Hill Lions 45 | † |
| 10/9/2010 | Newberry Wolves 63 @ Tusculum 56 | † |
| 10/16/2010 | Tusculum 27 @ Wingate Bulldogs 33 | † |
| 10/23/2010 | Tusculum 31 @ Lenoir-Rhyne Bears 34 | † |
| 10/30/2010 | Brevard Tornados 35 @ Tusculum 51 | † |
| 11/6/2010 | Tusculum 54 @ Catawba Indians 44 | † |
| 11/13/2010 | Carson-Newman Eagles 49 @ Tusculum 48 | † |