| 9/2/2010 | Mayville State 6 @ Minnesota-Crookston Golden Eagles 37 | |
| 9/11/2010 | Mayville State 31 @ Trinity Bible Lions 0 | |
| 9/18/2010 | Mayville State 10 @ Minot State Beavers 42 | † |
| 9/25/2010 | Black Hills State Yellow Jackets 20 @ Mayville State 7 | † |
| 10/2/2010 | Mayville State 10 @ South Dakota Tech Jackrabbits 58 | † |
| 10/9/2010 | Dickinson State Blue Hawks 37 @ Mayville State 7 | † |
| 10/16/2010 | Mayville State 7 @ Jamestown Jimmies 44 | † |
| 10/23/2010 | Valley City State Vikings 36 @ Mayville State 6 | † |
| 10/30/2010 | Dakota State Trojans 14 @ Mayville State 21 | † |