| 9/4/2010 | Benedict Tigers 18 @ Tuskegee 34 | † |
| 9/11/2010 | Tuskegee 41 @ Stillman Tigers 15 | † |
| 9/18/2010 | Albany State GA Golden Rams 34 @ Tuskegee 6 | † |
| 9/25/2010 | Texas Southern Tigers 14 vs Tuskegee 21 (Mobile AL) | |
| 10/2/2010 | Miles Golden Bears 0 @ Tuskegee 51 | † |
| 10/9/2010 | Morehouse Maroon Tigers 15 vs Tuskegee 31 (Columbus GA) | † |
| 10/16/2010 | Tuskegee 0 @ Fort Valley State Wildcats 26 | † |
| 10/23/2010 | Tuskegee 37 @ Clark Atlanta Panthers 10 | † |
| 10/30/2010 | Kentucky State Thorobreds 21 @ Tuskegee 45 | † |
| 11/6/2010 | Lane Dragons 6 @ Tuskegee 28 | † |
| 11/25/2010 | Tuskegee 17 @ Alabama State Hornets 10 |