| 9/5/2009 | Guilford 7 @ Greensboro 12 | |
| 9/12/2009 | Guilford 19 @ Methodist 20 | |
| 9/19/2009 | Averett 28 @ Guilford 3 | |
| 9/26/2009 | Hampden-Sydney 31 @ Guilford 6 | † |
| 10/2/2009 | Southern Virginia 6 @ Guilford 48 | |
| 10/17/2009 | Guilford 9 @ Bridgewater VA 40 | † |
| 10/24/2009 | Washington and Lee 28 @ Guilford 21 | † |
| 10/31/2009 | Randolph-Macon 13 @ Guilford 20 | † |
| 11/7/2009 | Guilford 42 @ Catholic 21 | † |
| 11/14/2009 | Emory & Henry 17 @ Guilford 3 | † |