| 8/29/2009 | Missouri Southern 15 @ Harding 17 | |
| 9/12/2009 | Missouri Southern 31 @ Washburn 54 | † |
| 9/19/2009 | Emporia St 13 @ Missouri Southern 34 | † |
| 9/26/2009 | Nebraska-Omaha 44 @ Missouri Southern 36 | † |
| 10/3/2009 | Pittsburg St 21 @ Missouri Southern 13 | † |
| 10/10/2009 | Missouri Southern 6 @ NW Missouri St 52 | † |
| 10/17/2009 | Truman St 16 @ Missouri Southern 20 | † |
| 10/24/2009 | Missouri Southern 35 @ Missouri Western 34 | † |
| 10/31/2009 | Missouri Southern 17 @ Central Missouri 27 | † |
| 11/7/2009 | Fort Hays St 48 @ Missouri Southern 34 | † |