| 9/5/2009 | Jamestown 11 @ Peru St 23 | |
| 9/12/2009 | UW-Stout 24 @ Jamestown 14 | |
| 9/19/2009 | Dickinson St 19 @ Jamestown 7 | † |
| 9/26/2009 | Jamestown 7 @ Minot St 33 | † |
| 10/3/2009 | South Dakota Tech 33 @ Jamestown 42 | † |
| 10/10/2009 | Jamestown 49 @ Mayville St 13 | † |
| 10/17/2009 | Dakota St 21 @ Jamestown 55 | † |
| 10/24/2009 | Jamestown 30 @ Black Hills St 21 | † |
| 10/31/2009 | Jamestown 38 @ Valley City St 19 | † |
| 11/8/2009 | Minot St 38 vs Jamestown 21 (Fargo ND) | † |