| 8/22/2009 | Tuskegee 27 @ Benedict 7 | † |
| 9/6/2009 | Tuskegee 16 vs Miles 23 (Birmingham AL) | † |
| 9/12/2009 | Fort Valley St 15 @ Tuskegee 22 | † |
| 9/19/2009 | Clark Atlanta 21 @ Tuskegee 31 | † |
| 9/26/2009 | Tuskegee 35 vs Kentucky St 28 (St Louis MO) | † |
| 10/3/2009 | Alabama A&M 35 vs Tuskegee 15 (Indianapolis IN) | |
| 10/10/2009 | Tuskegee 30 vs Morehouse 16 (Columbus GA) | † |
| 10/24/2009 | Tuskegee 21 @ Albany St GA 19 | † |
| 10/31/2009 | Tuskegee 40 @ Lane 6 | † |
| 11/7/2009 | Stillman 14 @ Tuskegee 41 | † |
| 11/26/2009 | Tuskegee 21 @ Alabama St 0 | |
| 12/5/2009 | Elizabeth City St 7 @ Tuskegee 21 |